SeGFClass (Secondary Structure Genetic Family Clasificator) is a tool which allows you to classify genes with previously created profiles that represent well known gene families. The SeGFClass algorithm is based on secondary structure protein alignments. As input, it needs the amino acid sequences that represent the proteins encoded by your genes of interest. This tool is divided in three sections: 1. Create a new personal Profile Upload a file with proteins in FASTA format (of a well known gene family) and a name for the profile. You will receive by email a zip file which contains this profile in a suitable format for our tool. 2. Upload personal Profile Here we can upload our personal profiles (the ones you receive by email) for a later usage. 3. Classify your own sequences according to a public profile or our uploaded personal profile Here we can classify the sequences using public profiles or personal profiles obtained in steps 1 and 2. In SeGFClass results, each row represents one of the input sequences, and each column represents a profile subfamily used for classification. Scores represent the sequence alignment quality for each column subfamily. Green scores are considered as matches. If in one row there is more than one match, we will consider that this sequence is very similar to the profile you are working with. Computational time for sections 1 and 3 is proportional to the number of input proteins. SeGFClass can approximately obtain results in 3 minutes for a single protein. Therefore, for more than 500 proteins, computational time may be specially long (at least one day to receive the results).

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